Coding

Part:BBa_K5226043:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-22)


stth


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1084
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 213
    Illegal NgoMIV site found at 471
    Illegal NgoMIV site found at 1174
    Illegal NgoMIV site found at 1440
    Illegal AgeI site found at 359
    Illegal AgeI site found at 700
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation.The base pairs at 183 bp and 555 bp were changed from a/t to g/c, and the base pairs at 444 bp and 345 bp were changed from g/c to a/t.

Source

Synthetic（BGI）. Sequence source is from Streptomyces toxytricini, found through NCBI （https://www.ncbi.nlm.nih.gov/protein/E9P162.1）

References